Dictyostelium discoideum : a New Method for Cloning in Liquid Medium
نویسندگان
چکیده
منابع مشابه
Dictyostelium discoideum: a new method for cloning in liquid medium.
Dictyostelium discoideum can be cloned in droplets of liquid medium by a method that permits observation of the complete life cycle. This alternative to solidified media provides advantages in clonal analysis.
متن کاملA defined minimal medium for axenic strains of Dictyostelium discoideum.
A defined, minimal medium for the cellular slime mold Dictyostelium discoideum is reported.
متن کاملCloning and Characterization of Replication Protein A from Dictyostelium discoideum
Charles L. Rutherford, Pawjai Khampang, Xiao Wen, Reyna Favis and Ian McCffery. Purification and Identification of a Protein that Binds to Regulatory Sites of the Glycogen Phosphorylase-2 Promoter of Dictyosteliym discoideum . International Dictyostelium Conference, October 13-18, 1996, Sendai, Japan
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Dictyostelium discoideum is a powerful and genetically tractable model system used for the study of numerous cellular molecular mechanisms including chemotaxis, phagocytosis and signal transduction. The past 2 years have seen a significant expansion in the scope and accessibility of online resources for Dictyostelium. Recent advances have focused on the development of a new comprehensive online...
متن کاملStationary phase and the cell cycle of Dictyostelium discoideum in liquid nutrient medium.
Cells of the axenic strain of the cellular slime mould Dictyostelium discoideum, AX-3, multiply in the liquid nutrient medium HL-5 with a doubling time of 12 h. When the cell concentration reaches approximately 1 X10(7) per ml the rate of cell multiplication begins decreasing and after 20-30 h reaches zero, at a stationary phase cell concentration of 2 to 2-5 X 10(7) cells per ml. The intercept...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1974
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.117.3.1363-1364.1974